NEBcutter
	Help for the Enzyme List page

This page provides a list of enzymes cutting or not cutting your sequence, based on the criteria you can specify by selecting a relational symbol (<=, =, >=) and entering the desired number of cuts. After hitting the OK button the appropriate enzymes will be listed along with their exact cut positions (if there are any). If the enzyme cuts more than five times then the link "see list" is provided to see the full list of the enzyme's cuts.

The cut coordinates are colored based on the end produced. For sticky ends the cut position on both the forward and complementary strands are shown in the form: forward cut / complementary cut. The number corresponds to the nucleotide after which cleavage takes place. Ambiguous sites are marked by putting their coordinates in parentheses. Sites that carry an  *  or  #  are susceptible to DNA methylation of one or more bases within their recognition sequence. Enzymes shown in purple are obtainable from NEB, those in blue from other commercial suppliers.

#  means that the enzyme is susceptible to methylation by one of the common E. coli methylases, Dam (GATC), Dcm (CCWGG), EcoBI (TGANNNNNNNNTGCT) or EcoKI (AACNNNNNNGTGC).

*  means that the enzyme is susceptible to CpG methylation as is found in many DNAs from higher organisms, especially mammals.

In the "Sites & flanks" column a link is provided to list all sites of the given enzyme along with the flanking sequences and cut sites marked.

If it's applicable then a "Sort order" selection box is provided where you can select how you want the list to be ordered.

Use the "Save as text file" button to save the currently displayed table as a plain text file to your disk.