Try the updated NEBcutter V3.0
This tool will take a DNA sequence and find the large, non-overlapping open
reading frames using the E.coli genetic code and the sites for all Type II
and commercially available Type III
restriction enzymes that cut the sequence just once. By default, only enzymes
available from NEB are used, but other sets may be chosen. Just enter your
sequence and "submit". Further options will appear with the output.
The maximum size of the input file is 1 MByte,
and the maximum sequence length is 300 KBases.
Warning: your browser is not fully supported by NEBcutter.
To achieve full functionality please use
Netscape Navigator 4.0 or higher, or Microsoft Internet
Explorer 4.0 or higher.
What's new in V2.0