[NEB homepage]

NEBcutter V2.0

Try the updated NEBcutter V3.0



This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut the sequence just once. By default, only enzymes available from NEB are used, but other sets may be chosen. Just enter your sequence and "submit". Further options will appear with the output. The maximum size of the input file is 1 MByte, and the maximum sequence length is 300 KBases.

Warning: your browser is not fully supported by NEBcutter. To achieve full functionality please use Netscape Navigator 4.0 or higher, or Microsoft Internet Explorer 4.0 or higher.
What's new in V2.0     Citing NEBcutter
Local sequence file:
Standard sequences:
GenBank number:    [Browse GenBank]
or paste in your DNA sequence:   (plain or FASTA format)
The sequence is:   Linear
Enzymes to use:   NEB enzymes
 All commercially available specificities
 All specificities
 All + defined oligonucleotide sequences
 Only defined oligonucleotide sequences
       [define oligos]
Minimum ORF length to display: a.a.
Name of sequence:    (optional)

Earlier projects:

Note: Your earlier projects will be deleted 2 days after they were last accessed.
You need to have cookies enabled in your browser for this feature to work.

Disable NEBcutter cookies